A Sustainable analysis of Comparative Genomics to study of Antioxidant Compound

Abstract

Neurodegenerative disorders, including Alzheimer’s disease, Parkinson’s disease and Huntington’s disease, present in a major health issue and the financial load for the health care systems and around the world. The impact of these the diseases will be further increase and decrease over the next decades caused by the increasing life expectancies of the health. No cure is currently available for the treatments of the these conditions; only drugs, which are merely alleviate the symptoms. Oxidative stress has been lengthy been associated with a neuro degeneration, whether as a cause or as part of the downstream and upstream results caused by the other factors and other effects. Thus, the use of the antioxidants to the counter cellulars and a oxidative stress and antioxidant within the nervous system has been suggested as a potential treatments option for the neurological disorders. Over the last decade, it has to be significant research has to be the found to be most and focused on the potential use of natural antioxidants to be target oxidative stress. However, clinical trial results have been lacked success for the treatment of the patients with neurological disorders. The knowledge that natural extracts shows other positive molecular activities in addition to the antioxidant activity, however, has led to further research of natural extracts for their potential use as well as prevention or treatment/management of the neurodegenerative diseases. The diversity of the wild mushrooms was a investigated from two protected forest areas in India and 232 mushroom specimens were morphologically identified. Among them, 77 isolates were screened for the antimicrobial potential against seven and bacterial and fungal pathogens. Out of the 77 isolates, 46 isolates which displayed significant antimicrobial activities were identified using ITS rRNA gene amplification and subsequently phylogenetically characterized using a random amplified polymorphic DNA (RAPD) and inter-simple sequences repeat (ISSR) markers. Sequencing of the ITS rRNA regions classified the isolates into 16 genera belonging to the 11 families. In total, 11 RAPD and 10 ISSR primers were selected to be the evaluate genetic diversity based on the their banding profile produced. In total 337 RAPD and 312 ISSR bands were to be detected, among which is percentage of the polymorphism ranges from 35.2% to 77.8% and 37.6% to 93.4% by using RAPD and ISSR primers respectively. The report has to be also demonstrated that both RAPD and ISSR could be efficiently differentiate wild mushrooms and it could thus be the considered as the efficient markers for the surveying genetic diversity. Additionally, selected six or more then six wild edible mushroom strains (Schizophyllum commune BPSM01, Panusgiganteus BPSM27, Pleurotussp. BPSM34, Lentinussp. BPSM37, Pleurotusdjamor BPSM41 and Lentinula sp. BPSM45) were a analyzed for the their nutritional (proteins, carbohydrates, fat and ash content), antioxidant potential.