In Vitro analysis of the Antibacterial action of the Extract of Costus Spiralis (Costaceae) on Enterococcus Faecalis

Abstract

Enterococcus faecalis is an important cause of endodontic and nosocomial infections, making the search for new antimicrobial drugs important. To analyze in vitro the antibacterial action of Costus spiralis extract on E. faecalis. The antimicrobial sensitivity test was performed using the well agar diffusion method. E. faecalis strains (NEWProv-0012) were revitalized in BHI broth and incubated at 37 ° C for 18 h. Subsequently, the microbial suspension was adjusted to a concentration of 5x105 CFU mL-1 and sown on Mueller Hinton agar, after which 6 mm wells were made. The crude alcoholic extract paste of C. spiralis was used as a positive control 2.5% sodium hypochlorite and as a negative control 96% ethanol and BHI broth. The halos of minimal inhibitory inhibition (MIC) using the crude extract paste and solutions prepared with BHI broth in different concentrations. The viability and microbial quantification of E. faecalis in culture with MIC were determined by serial dilution after 24, 48 and 72 h. Results: The crude extract of C. spiralis has an effective action in inhibiting the growth of E. faecalis. MIC was observed at a concentration of 25% of the extract in solution and promoted the inhibition of E. faecalis growth by 50% after 24 h and 98% in 48 h of incubation. Conclusion: The C. spiralis extract has antibacterial action and can be a therapeutic alternative for infections caused by E. faecalis.