Antifungal Susceptibility Pattern against Dermatophytic Strains Isolated from Humans in Anambra State, Nigeria

Abstract

Dermatophytosis are fungal infection that involve only superficial keratinized tissue of the body, skin, hair and nails. Out of the 1000 cultured samples on Sabroud dextrose agar, 320(32%) isolates of dermatophytes were isolated. Susceptibility testing was performed using sterilized discs (6mm) prepared from whatman No. 1 filter paper, impregnated with different concentrations (25mg, 50mg, 100mg and 200mg) of terbinafine, itraconazole, ketoconazole, fluconazole and griseofulvin dissolved in 2% dimethylsuphuroxide (DMSO).These dermatophytes tested were susceptible to the five antifungal drugs used. The MIC of the quality control strains was within established ranges. However no resistance was recorded among the isolates. The isolates were less susceptible to griseofulvin and fluconazole with MICs ranging from 0.1 – 4.00 and 0.007 – 0.500ug/ml respectively. The MIC range for them respectively is between 0.06 – 0.125ug/ml for griseofulvin and 4.0 – 16.0ug/ml for fluconazole. Most dermatophytes 320(32%) were susceptible to terbinafine, since the MIC range was between 4.0 – 16.0 ug/ml. The study showed that there is no abuse of antifungal drugs in this area whose samples were used for the study.