Bacterial wilt resistant gene searching in Eggplant (Solanum melongena) and its two wild relatives

Abstract

Eggplant is an important vegetable in all over the world. Bacterial wilt caused by Ralstonia solanacearum is a major disease of eggplant. Near about 32% crop loss occurred due to this disease. The wild relatives of eggplant viz. Solanum villosum and Solanum sisymbriifolium showed resistant against bacterial wilt disease. Hence, attempted was made to search the resistant gene of bacterial wilt in three Solanum spp. Some total of 16 different bacterial wilt resistant molecular markers RAPD (OPB-17, OPG-05, OPH-07) UBC (176, 205, 317) SSR (STM0007, emh01J23, emb01N07) SCAR (me1, me2, me4, me5) and SRAP (SCU176-534, SCU176-1190F1R1, SCU176-1190F2R2) were screened to identified the resistant DNA fragment. Only one primer UBC#176 showed amplification in target DNA. The primer UBC#176 gave DNA amplification in target position at 350 bp in the species of Solanum villosum. The obtained DNA fragment showed maximum 79% homology with Solanum lycopersicum cultivar I-3 chromosome 8 which has GeneBank Accession No. CP023764.1. Alternative approach was made to identify resistant gene of bacterial wilt disease. Total RNA was extracted and cDNA was synthesized. The synthesized cDNA was used as template to find out the resistant gene. But none of the gene specific primer was amplified using cDNA as template. Further study was needed to find out the bacterial wilt resistant DNA fragment in wild type.