Comparison the Concentration of Purification Antigen MTSP11 and MPT63 as Serodiagnostic Active Tuberculosis

Abstract

Tuberculosis remains a global concern in many countries because it is a contagious disease and become the second largest killer after HIV / AIDS. In suppressing the number of deaths from TB disease are increasing from year to year we conducted this test target antigen of Mycobacterium tuberculosis that is immunogenic by using molecular biology techniques. This study aims to the purification of recombinant proteins MTSP11 and MPT63 measuring the concentration of purified protein as a candidate serodiagnostic active tuberculosis. E. coli cells carrying the recombinant plasmid pQE30 Xa-Rv 3204 and pQE30 Xa-Rv 1926 cultured in LB medium containing ampicillin, then, do cell lysis. Further characterization of proteins and the last stage is the measurement results of protein purification. The results obtained are MTSP11 protein has a molecular weight of 11 kDa while the MPT63 protein size of 16 kDa. The result of the calculation using the formula showed that the concentration of MTSP11 0.165423045mg / mL and the concentration of MPT63 is 0.155164115 mg / mL, the concentration of purified protein is best found in the last washing results by using the elution buffer.