Determination of The Presence of Brown Planthopper Resistance Genes (Nilaparvata lugens Stål.) in Rice (Oryza sativa L.)

Abstract

The main goal of this current study is to determine the presence of brown planthopper (BPH)-resistance genes in some rice varieties to provide initial materials for the breeding program of BPH resistant rice varieties. According to the investigation, several molecular markers were used, such as RM1103, RM204, RM217, RM545, and RM401; and associated with BPH resistance genes Bph1, Bph3, bph4, Bph13, and Bph17. Our study was conducted in the laboratory of molecular genetics, the greenhouse, the field trials in CLRRI, and the laboratory of PCR and Biotechnology Company. The outcome indicated that there were 10 varieties showed with sustained resistance to some BPH populations in the Mekong Delta; a few indicator-resistance and indicator-susceptible varieties were assessed the genotyping through Simple Sequence Repeat (SSR) markers. Furthermore, they showed the presence of genes in the varieties such as TLR493 (Bph1), OM7268, OM6830, OM10279 (Bph3), OM6683, and Tau Huong (Bph1, Bph3, and Bph13), OM7364 (Bph1, bph4, and Bph13), OM5954 (Bph1 and Bph13), Chom bok Khmum (Bph3 and Bph17), but the Chet Cut variety was not showing the presence of five genes.