The impact of using theZingiber officinale extract in controlling the endogenous bacterial contamination of date palm during tissue cultures.

Abstract

Endogenous bacterial infections are the most critical problem hampers date palm (Phoenix dactylifera) micropropagation cycle. This work aimed to evaluate the influence of the antibacterial property of ginger(Zingiber officinale) extract on suppressing the endogenous bacterial contamination of date palm tissuecultures, to avoid the continuous using of the ordinary chemical antibiotic. The prepared aqueous extract ofZingiberofficinaleat different concentrations (0.5, 1, 2, 4, 6, 8, or 10 ml/l), in comparison toantibiotic, Chloramphenicol or Tetracycline at 100 mg/l, studied onmultiplication media of Gondelah cultivar.The residual effect of all studied treatments also followed up on the rooting media. The results showed that the concentrations of ginger extract at 8 or 10 ml/l (without significant result between) came in the second rank follow the Tetracycline in inhibition the growth of the endogenous bacterial contamination of shoot clusters during the multiplication stage, but subsequently, their residual effect during the rooting stage recorded the highest significant results over the use of antibiotics in preventing bacterial growth in plantlets’ cultures. Ginger extract at 8 or 10 ml/l gave the highest significant results of the vegetative growth of shoot clusters explants on multiplication media, as well as, the highest values of plantlets vegetative growth during the rooting stage. So, using the ginger extract as an organic antibacterial source for controlling the endogenous bacterial problem of the date palmensures that cultures are controlled protective, well vegetative growth, and avoiding the disadvantages of the continuously using of ordinary antibiotics.