Antibacterial Activity Rhizophora stylosa and Avicennia marina of Mangrove Fruit Extraction on Vibriosis of Mangrove Crab Larvae (Scylla Serrata Forsskal)

Abstract

The stability of mangrove crabs (Scylla serrata Forsska0l) production were still constrained, especially the high mortality in the larval stage caused by vibriosis infection in the form of vibrio bacterial attack, so natural extracts as antibacterial such as R.stylosa and A.marina were needed. The purpose of this study was to assess the antibacterial activity R.Stylosa and A.marina of mangrove fruit extracts through in-vitro as the cause vibriosis of mangrove crab larvae. (Scylla serrata F). On fruit sample and extraction of active ingredients R.Stylosa and A.marina fruit were the initial stages of the study, then continued with bacterial isolation, soaked calculation of% and antibacterial activity test for antibacterial on vibrio bacteria. The results of the soaked calculation of% R.stylosa at the concentration of 2 mg / disk / 50 μl, the highest obtained in metanol was 12.92% and A.marina was 8.61% in metanol; The results of antibacterial activity based on inhibition zone area, for the highest R.stylosa in solvent metanol (11.40 mm) , in V.harveyi bacteria, then followed by 7.18 mm in V. alginolyticus bacteria, then 7.03 mm in V.paraemolyticus bacteria, where as the results of antibacterial activity based on inhibition zone area, for the highest A.marina in the Chloroform solvent (21.09mm), in V.harveyi bacteria, then followed 19.08mm in V. paraemolyticus bacteria, and 11.80mm in V.alginolyticus bacteria. Obstacle zones obtained categorized as moderate to very high (> 11- <15mm = moderate; height = 15-20 mm; and very high => 20mm) based on the description at Zainuddin (2006) so that they met the requirements to be applied through in-vivo.