The Effect of Some Verbascum Plant Extracts on Cytoplasmic Membrane of Multidrug Resistant Bacteria by Flow Cytometry

Abstract

Verbascum species have been the focus of antioxidant and antimicrobial studies thanks to their secondary metabolites, especially saponins. While disk diffusion and dilution methods are generally used within the scope of antimicrobial studies, the Flow cytometry method is not well known. The ability of six Verbascum species to increase the permeability of multidrug resistant bacterial cells was conducted by flow cytometric assay on Listeria innocua and Escherichia coli. Fluorescence based flow cytometry is a technique for measuring characteristics of cells or biological particles and using flourescent dye as fluorogenic substrate provides. Propidium iodide (PI) is a fluorescent molecule and it can be used to stain cells. The SYTO dyes can be used to stain nucleic acids in both live and dead cells, as well as in Gram-positive and Gram-negative bacteria.