Quality Improvement of Durian Waste and Tofu Waste Fermented with Pleurotus ostreatus

Abstract

This study was conducted to determine the dose of inoculum and the duration of fermentation that is appropriate for the growth of Pleurotus ostreatus on a mixed substrate fermented of durian waste and tofu waste (DWTW) on crude fiber content, crude protein content and cellulase enzyme activity. This research was designed using Factorial Completely Randomized Design 3x3 with 2 replications. Factor A consisted of the dose of inoculum A1 (6%), A2 (8%), A3 (10%) and Factor B consisted of the duration of fermentation namely B1 (7 days), B2 (9 days), B3 (11 days). The variables observed were crude fiber content, crude protein content and cellulase enzyme activity. The results of the analysis showed that there was an interaction between the inoculum dose and the length of DWTW fermentation with Pleurotus ostreatus which had a significantly different effect (P <0.05) on cellulase enzyme activity, crude fiber content, and crude protein content. From the results of this study it can be concluded that the 8% inoculum dose and 9 day fermentation time are optimal and efficient conditions for increasing the quality of the mixture of durian fruit and fermented tofu waste with Pleurotus ostreatus. In this condition cellulase enzyme activity was 1.36 U / ml, crude fiber content was 14.69%, and crude protein content was 19.25%.