In vitro Tissue Culture Technique as Tool for micro propagation of Lepidium sativum L. Plant under Abiotic Stress Conditions

Abstract

This study was carried out at biology Lab, Biology Department, University College in ALKhafji, University of HAFR ALBATIN, to study the effect of MS salts strength, and sucrose concentration on micro propagation and phytochemical composition of Lepidium sativum Plant, using MS medium at five salt strength (1.0,1.5,2.0,2.5 and 3.0 mg\L) MS salt strength and sucrose at five concentration (30.0 g, 45.0 g, 60.0 g, 75.0 g and 90.0 g) \L. Ethanolic extracts and DPPH reagent were used to test the antioxidant activity. Lepidium sativum Plant used in folk medicine in Sudai Arabia, known as Garden cress belongs to Brassicaceae family. It is very famous in folk medicine. The tissue culture technique is a rapid method to propagate plants in vitro, especially medicinal plants for producing medicinal component. Through in vitro culture we can control the environmental conditions of plants; it enables us to study the effect of some factors like sucrose concentration and salt strength that determine growth of plants and chemical constituent. Impact of these stresses the plant micro propagation and the content of plants from active substances which plays a role as antioxidant was varied. For micro propagation, the effect of MS salt strength showed that, the use of MS media salt at double MS salt concentration (2.0 MS salt) obtained highest leaf number (8.11±1.3278), highest node number was (1.933±0.62098) and highest shoot length (4.608±0.5828cm) both were induced by basal MS (1.0 MS salt), while highest root length (3.787±0.3958) obtained at triple MS salt strength (3.0 MS salt (. Effect of sucrose concentration, MS media supplemented by 30 g sucrose obtained highest leaf number, highest shoot and root length (5.655±1.1076; 4.608 ±0.5828 and 2.985±0.3952) respectively. For antioxidant activity data represented that antioxidant activity increase with increasing salt strength and sucrose concentration. Our results suggested that increasing sucrose concentration decrease leaf number, shoot and root length, on the other hand increase antioxidant activity. While increasing MS salt strength decrease node number and shoots length, while root length and antioxidant activity was increase. The results contained in this research are the first study to Effect of some environmental stress on in vitro propagation and antioxidant activity of Lepidium sativum at Saudia Arabia.