Extraction of Catechins from Areca catechu L.Peel with different Solvent Type for Feed Additive of Broiler

Abstract

Abstract: Catechins are secondary metabolites compound of flavonoids group that is naturally produced by plants and have health benefits as cholesterol-lowering, antioxidant, antimicrobial in mice.Rapid growth in broilers is often followed by high-fat growth as well, thus causing a high cholesterol content in the broiler's meat especially in thigh meatand wings.Feeding extract catechin in broiler as feed additive will reduce cholesterol or fat in broiler’s meat. The catechins could be extracted by maceration method, so far there is no information about the type of solvent and extraction method for extraction of catechin from Areca catechu peel. The aims of this study to obtain the best combination of solvent type and maceration duration in extracting of catechins from betel nut peel. Areca catechu peel used in this study is a green-colored fruit peel, which washarvested from betel nut seeds in Batu Balang village, Lima Puluh Kota District, West Sumatra Province, Indonesia. This study was performed by using Factorial Experimental in Completely Randomized Design withtwo factors. The first factors was solvent types (water with an initial temperature at 80oC, acetone distillate, and ethyl acetate distillate) and the second factors wasmaceration duration (6 hours, 12 hours, and 18 hours), each combination treatment was replicated 3 times.The variables observed were percentage of water content, percentage of yield, and percentage of total catechins. The results showed there was a significant interaction (P <0.05) between type of solvent and maceration duration on yield percentage andtotal catechins percentage, while there was no interaction betweentype of solvent and maceration duration(P> 0.05) on percentage ofwater content. Both type of solvent and maceration duration were significantly (P <0.05) affectedyield percentage andtotal catechins percentage, whilepercentage ofwater content did not affect. It concluded the combination of distillate acetone solvent and maceration duration for 6 hours was the best combination to obtain catechin extract from Areca catechu peel. In this condition the percentage of water content was 10.53%, yield percentage was 7.13%, and percentage of catechin extract was 25.53%.