Antibacterial Activities of Lactic Acid Bacteria from Langsat Fruit (Lansiumdomesticum) against Phatogenic Bacteria and Spoilage Bacteria

Abstract

Abstract: Langsat fruit (Lansiumdomesticum) sample collected from 4 district in Minahasa, North Celebes. Lactic Acid Bacteria (LAB) isolates were found at that samples. The study aim to examinedthe antibacterial activity of Lactic acid bacteria (LAB) from Langsat fruit sample against pathogenic bacteria Escherichia coli (Gram Negatrive Bacteria) and spoilage bacteria Staphylococcus aureus (Gram Positive Bacteria) using agar well diffusion method.The isolation result obtained 42 isolates of LAB namely 10 isolate were obtained from Southeast Minahasa district, 8 isolates were obtained from the southern district of Minahasa, 12 isolates were obtained from from the Minahasa district, and 12 isolates were obtained from North Minahasa district. Fourty two isolates LAB identified as Lactobacillus genera based on profile matching method were effective against bacteria indicator (pathogenic and spoilage bacteria). The antibacterial activity showed through the formation of a clear zone around well that containing LAB isolates. The diameter inhibition of LAB isolates ranged from 3,0 – 17,0 mm. Overall, the isolated LAB showed the remarkable inhibitory effect against both Gram positive (spoilage bacteria) and Gram negative (pathogenic bacteria). However, the spectrum of inhibition was different for the isolates tested. These results suggest that this potent isolates could be used as a natural biopreservativesagent.