In-Vitro Efficacy of Commercial Fungicides against Bipolaris Sorokiniana: Induced Spot Blotch Disease of Wheat

Abstract

Spot blotch caused by Bipolaris sorokiniana is an important disease of wheat. A laboratory experiment was conducted at Plant Pathology Division, Nepal Agriculture Research Council, Khumaltar, Nepal to evaluate the efficacy of commercially available fungicides viz, Saaf ( Carbendazim 12%+ Mancozeb63%), Sectin (Fenamidone 10%+ Mancozeb 50%), Angel (Metalaxyl 8%+ Mancozeb 64%), Diathane M-45 (Mancozeb 75%), G-MIL( Cymoxanil 8%+ Mancozeb 64%) tested in two different concentration i.e. 50ppm and 100 ppm except Curex (Copper oxychloride 50%) tested in the concentration of 100ppm and 200ppm and Tilt (propiconazole 25%) tested in 15ppm and 30 ppm concentration with a control test by employing poisoned food technique against spot blotch pathogen of wheat Bipolaris sorokiniana. The experiment was laid out in Completely Randomized Design (CRD). The result revealed that all concentrations of different fungicides successfully inhibited the radial mycelial growth of the pathogen under in vitro condition. Based on the measurement of fungal radial growth, fungicide Tilt of 30ppm and 15 ppm concentration were the most effective followed by Sectin 100ppm, Diathane M-45 100ppm, Angel 100 ppm and Saaf 100ppm. G-MIL 50 ppm in poisoned food technique was the least effective. Use of safer and economical chemical fungicides can provide an effective and long-term solution against plant diseases in agricultural farming.