Antifeedant test extracts of Hutun seeds against caterpillar pests Plutella xylostella on Sawi Plant

Abstract

Application of the bioactive extract of the hutun seed (Barringtonia asiatica L.kurz) as an antifeedant in the Plutella xylostella Lcaterpillar pest on sawi plants ( Brassica juncea L.) was carried out. This study aims to determine the antifeedant activity of the hutun seed extract in controlling the caterpillar sawi Plutella xylostella. The method used in this research is a completely randomized design (CRD) method with 4 treatments and 3 repetitions. The concentrations used are 50 ppm, 100 ppm, 500 ppm and 1000 ppm as well as positive control and negative control. The results of the study were tested with one-way ANOVA and continued with the Least Significant Difference test (LSD). Results of the study show that there are differences antifeedant activity caterpillar pests Plutella xylostella on sawi plants that significant at different levels of concentration. The study was conducted in several stages starting from the stage of extraction of the hutun seed, phytochemical test, preparation of caterpillar test, antifeedant activity testing. As the treatment is the level of methanol concentration of 50 ppm, 100 ppm, 500 ppm and 1000 ppm. The parameter observed was the percentage of Feeding Reduction (FR). The test results showed antifeedant activity against caterpillars of Plutella xylostella supreme contained at a concentration of 500 ppm and 1000 ppm because it can hinder eating caterpillars test amounted to 27.80 % and 40.93 %.