Detection molecular for microdeletions of the Y chromosome in IVF technique

Abstract

Y chromosome microdeletion tests are the basis of success IVF technique to disclose the real problem for patients males (oligozoospermia) to reproductive treatment. The present study to showed the relationship between microdeletion and IVF technique, then early detection of Y chromosome microdeletion is auxiliary to venereal treatment, particularly in those young patients with AZFc deletion. The study was used 65 patients, males (oligozoospermia ) and ten normal males, as control. DNA was recovered and treated, which was used for Y (CMD) through PCR SYBR Green real-time PCR for the amplification of three loci AZFa, AZFb, and AZFc regions. Our results showed only six samples (9 %) of single micro-deletions in the Y chromosome. Finally, the early diagnosis is very important for oligozoospermia patients whose will undergo the IVF technique. Kew word: Y chromosome, IVF technique, SYBER Green, Oligozoospermia.