Synthesis & Pharmacological Activity of Flow Cytometry Evaluation of Apoptosis

Abstract

The specific morphological and biochemical hallmarks of an apoptosing cell are very context- and stimulus-dependent. Flow and image cytometry can quickly analyze the vast majority of the conventional apoptotic markers. As a result, cytometry evolved became a go-to method for several investigations of cell death. Over the last two decades, several cytometric techniques have been developed to detect apoptotic cells and investigate the processes involved in this kind of cell death. Treatment of UACC62 (Melanoma) cells with the synthesized coumarin was followed by analysis of morphological alterations, membrane change, mitochondria membrane potential, and caspase-3 activity using a flow cytometer and Annexin V-PI staining, JC-1, and caspase-3 enzyme kits. Doxorubicin, a common chemotherapy drug, was used as a benchmark.