Potential of salicylic acid rhizobacteria indigenous chili which is able to suppress Bacterial

Abstract

Plant growth promoting rhizobacteria are a group of bacteria that actively colonize plant roots, increase plant growth and control plant pathogens. Some strains of rhizobacteria can produce salicylic acid and are responsible for the induction of ISR in plants. Salicylic acid is widely recognized as the key to plant resistance. This study aims to analyze the potential of salicylic acid from indigenous rhizobacteria isolates from the roots of chili plants. Rhizobacteria isolates were isolated from the roots of healthy chili plants and endemic bacterial wilt disease. The isolation results were tested in planta for the ability to control bacterial wilt disease (Ralstonia solanacearum). Salicylic acid levels were analyzed using the HPLC method. Results of the test twenty chili ingenus rizobakteria isolates were able to suppress the attack of bacterial wilt. The analysis showed that only nine of the twenty isolates contained salicylic RZ.1.1.AG4, RZ.1.1AP1, RZ.1.2.AP1, RZ.1.3.AG4, RZ.2.1.AG1, RZ.2.1.AP1, RZ.2.1.AP3, RZ.2.1.AP4, dan RZ.2.2.AG2. Eleven other isolates namely namely RZ.1.3.AP1, RZ.1.4AG4, RZ1.4.AP4, RZ.2.1.AP2, RZ.1.5.AP4, RZ.2.3AG4, RZ.2.1.AG3, RZ.2.2.AG4, RZ.2.5.AP4, RZ.2.1.AG2 and RZ.2.3.AP1 do not produce salicylic acid. The highest concentration of salicylic acid from isolate RZ.1.1.AG4 at 20,95 ppm followed by RZ.1.1AP1 at 19.27 ppm, RZ.1.2.AP1 at 18.05 ppm, RZ.1.3.AG4 at 16.96 ppm, RZ.2.1.AG1 at 15.45, RZ.2.1.AP1 at 15.25, RZ.2.1.AP3 at 14.28, RZ.2.1.AP4 at 14.09, and RZ.2.2.AG2 at 13.45 ppm.