Mechanism of Indigenous Rhizobacteria Isolate Growth Inhibition of Ganoderma boninense

Abstract

Basal stem rot (Ganoderma boninense) is the main disease of oil palm. Previous research results obtained 7 isolates of rhizobacteria indigenous which have the best ability to control G. boninense in oil palm seedlings and increase growth. The purpose of this study was to characterize the ability of PGPR and biocontrol of indigenous rhizobacteria isolates and inhibitory testing of G. boninense in vitro. Characterization methods include (Phosphate solubility, siderophore production, chitinase activity, hemolytic activity and dual culture test). The results showed four isolates of RZ1A 2.1, RZ2E 2.1, RZ1E 2.1, and RZ2B 1.1 were able to dissolve phosphate. only isolate RZ2B 1.1 produced siderophore. Two isolates produced chitinase RZ1E 2.1 and RZ2E 2.1. All isolates did not produce hemolysis. The best three isolates were obtained from RZ1E 2.1, RZ2E 2.1 and RZ2E 1.2 which have inhibitory properties against G.boninense.