The Effect of Combination of Picloram and Bap on the Calculation of Clean Plants (Postogemon cablin Benth)

Abstract

 This study aims to determine the best Picloram and BAP concentrations in InVitro patchouli callus formation. This experiment was conducted in November 2018 to January 2019 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang. This study used an experimental method with Completely Randomized Design (CRD) consisting of two factors. The first factor was the administration of Picloram concentration with 5 levels of treatment and the second factor was the administration of BAP concentrations with 5 levels of treatment. This experiment consisted of: This experiment consisted of Factor I: Picloram (A) Without Picloram (A1), Concentration of 1 mg / L (A2), Concentration of 2mg / L (A3), Concentration of 3 mg / L (A4), Concentration of 4mg / L (A5), Factor II: Concentration of BAP (S) Without BAP (S1), Concentration of 0.5 mg / L (S2), Concentration of 1 mg / L (S3), Concentration of 1.5 mg / L (S4) Concentration 2 mg / L (S5). The data obtained were analyzed using the F test at the level of 5%, if F count is greater than F table then the analysis is continued with the DMNRT test at the level of 5%. From the results of the study showed that the concentration of picloram had a significant effect on the growth of patchouli callus with an average callus appearance of 7 HST and percentage (%) of explants growing up to 100%. High picloram concentration did not significantly affect callus growth, 2 mg / l picloram + BAP concentration did not produce callus.